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T. C. Pokhriyal , A. S. Raturi , H. O. Nautiyal , S. R. Joshi

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T. C. Pokhriyal , B. C. Ramola , A. S. Raturi

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In the present study, seasonal changes in the moisture and N contents of the leaf, litter and soil upto 60 cm depth were carried out in the natural sal (Shorea robusta) forest, Jhajra, Dehra Dun. A significantly higher soil moisture contents were observed during the rainy seasons (July, Aug., Sept.) and lowest in the months of June. The maximum litter fall was observed in the months of April, March followed by May. The remaining months have poor litter fall and they do not differ among themselves signlficantly. The upper part of crown have significantly higher N contents as compared to middle and bottom. No slgnificant changes in the soil N was observed upto 60 cm depth. A negative correlation was observed between soil moisture and litter production rate and green leaf N and litter N. Whereas a positive correlation was noticed between soil N and litter production, this relationship was more pronounced during summer months.

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T. C. Pokhriyal , A. S. Raturi , S. P. Pant , S. K. Pande , S. K. Bhatnagar

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Nitrogen fixation studies were carried out in four tree species viz., Albizia lebbeck, Acacia nilotica, Dalbergia sisoo and Leucaena leucocephala under potculture conditions. Maximum plant height, nodule biomass, and nitrogenase (acetylene-ethylene reduction) activity was noticed in case of Leucaena followed by Albizia, Acacia and Dalbergia. The amount of n moles C₂ H₂, produced per plant h^-1 and per nodule h^-1 was 550.9 and 4.44 in Albizia, 488.88 and 4.40 in Acaica, 125 and 2.28 in Dalbergia and 1772.3 and 12.31 in Leucaena.

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A. S. Raturi , B. G. Dabral

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The paper details the water consumption behaviour or Chir-pine, Banj-Oak, Sal and Ipil-Ipil in Juvenile stage conducted in plastic and glazed earthen pots. Amongst Chir-pine, Oak and Sal, lowest transpiration ratio is depicted by Chir-pine and the highest by Oak. Although the transpiration ratio of Ipil-Ipil is lowest, its water consumption is highest. Ipil-Ipil being a rast growing species requires bigger space than that provided here for conducting the experiment. The results indicate that due to the comparatively bigger needs for water consumption by Chir-pine, replacement of degraded Oak forests by this species, on the northern aspects may dry the sites adversely, affecting the water regime of the tract but on southern and western aspects which are comparatively warmer and dryer the water regime may not be significantly affected. Thus it is thought that such degraded Oak areas in the hills, earmarked for Oak regeneration, should not be brought under Chir-pine, lest the already deficit soil moisture regime may further deteriorate. Besides, pines have a tendency to turn alkaline soils acidic, which is detrimental for Oak regeneration. However, studies are required to be done on the watersheds itself for confirmation. The plastic pots seem better indicative of treatment responses compared to the glazed earthen pots.

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T. C. Pokhriyal , A. S. Raturi

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The in vivo Nitrate Reductase activity was standardized for the leaflets of Leucaena leucocephala. The maximum enzyme activity was observed at 7.6 pH (0.05M) and 0.15 M Potassium nitrate concentration. During the study, maximum NR activity (mµ moles NO₃^- reduced g^-1 dry wt. h^-1) and total NR activity per leaf blade were estimated. In the upper leaf blades followed by a decrease in the subsequent leaf blades in the descending order. Fresh and dry weight values also followed almost the similar trend.

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T. C. Pokhriyal , A. S. Raturi

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The method for assay of in vivo nitrate reductase(NR) activity was standardised for poplar leaves. The maximium activity(mμ moles NO^-₃ reduced g fr, wt^-1h^-1)was obtained with a 0.05 m phosphate buffer of 7.6 pH and 0.05 m potassium nitrate concentration. Maximum NR activity and total activity(leaf)^-1 was observed in the sixth leaf from the tip and further it decreased with the increase in age. An increase in the fresh and dry weights of leaves was observed upto eighth leaf. The middle order leaves seem to be more efficient in the rate of photosynthesis to gain higher fresh and dry weights and NR activity as compared to young and old ones.

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A. S. Raturi , B. K. Subba Rao

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B. G. Dabral , A. S. Raturi

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The paper presents the results of water comsumption by Eucaluptus hybrid trees during the last 27 months in 3m × 3m × 3m R.C.C. lyalmeter. Water consuption form 30 to 24.5 cm depth was slightly lower than the rainfall received, although water consuption from the entire soil depth(0-24.5) exceeded the rainfall due to the previously stored soil water. Maximum amount of waater was consumed during rains and the least during summer.

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T. C. Pokhriyal , A. S. Raturi

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Accumulation of proline , a free amino acid is a common feature in some of the stress affected crop plants. An attemp was made to study the changes in the proline contents of diffferent leaflets of Eucalyptus hybrid. Maximum (608.77 μg/g dry wt.) and minimum (24.81 μg/g fr.wt.) proline cantents was estimated in the first and tenth leaf blades respectively. But the total proline per leaf blade was highest in the fourth and lowest in the first leaf blade.

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T. Pokhriyal , A. S. Raturi

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The method for assay of in vivo nitrate roduclase (NR) activity was standardised for for Eucalypllls leaf blades. The maximum activlly (m moles NO₃^- reduced g dr wt^-1 H^-1 was obtained with a 0.1 m phosphate buffer of pH 7.5 and 0.025 potassium nitrate concentration. Among the ten Individual lear blades (in descending order from the tip) maximum NR activity (m moles NO^-₃ reduced g dry wt^-1 H^-1) was obServed in the young (juvenile) ones, and further it dedreased with the increase in the age. Whereas, the total NR activity per leaf blade was higher in the middle order leaves (4th to 6th) and lowest in the tenth leaf. An increase in the fresh and dry weights of The leaves was observed upto seventh leaf, thereafter it remained almost constant.

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R. N. Gupta , P. G. Pattanath , Adarsii Kumar , R. C. Thapliyal , A. S. Raturi

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In order that results may be reproduceable, it is essential that germination tests for certification of seeds are standardised. The International Seed Testing Association has laid down guide lines for these tests in 1966, and based on these lines it is necessary to standardize the procedures for each species separately. In this connection, rules for germination tests of 55 Indian tree species have been worked out and tabulated.

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B. N. Gupta , A. S. Raturi

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This paper deals with the standardisation of viability test by tetrazolium staining in respect of six Indian tree species viz., Alibizia lebbeck, Albizia procera, Albizia chinensis, Cassia fistula, Cassia nodosa and Pinus roxburghii. Tetrazolium staining is a dependable and accepted biochemical method for testing the inherent germination capacity of a seed lot. This is especially important in forest seeds which take much longer time to germinate or exhibit varying types of dormancy. When soaked in TTZ solution, seeds receive red colour in various patterns depending upon the occurrence of living tissues in them. Attempt has been made to identify viable and non-viable categories of seeds and to standardize the method for their interpretation.

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